Tissue- and cell-type specific transcriptome profiling of expanding tomato fruit provides insights into metabolic and regulatory specialization and cuticle formation.
نویسندگان
چکیده
Tomato (Solanum lycopersicum) is the primary model for the study of fleshy fruits, and research in this species has elucidated many aspects of fruit physiology, development, and metabolism. However, most of these studies have involved homogenization of the fruit pericarp, with its many constituent cell types. Here, we describe the coupling of pyrosequencing technology with laser capture microdissection to characterize the transcriptomes of the five principal tissues of the pericarp from tomato fruits (outer and inner epidermal layers, collenchyma, parenchyma, and vascular tissues) at their maximal growth phase. A total of 20,976 high-quality expressed unigenes were identified, of which more than half were ubiquitous in their expression, while others were cell type specific or showed distinct expression patterns in specific tissues. The data provide new insights into the spatial distribution of many classes of regulatory and structural genes, including those involved in energy metabolism, source-sink relationships, secondary metabolite production, cell wall biology, and cuticle biogenesis. Finally, patterns of similar gene expression between tissues led to the characterization of a cuticle on the inner surface of the pericarp, demonstrating the utility of this approach as a platform for biological discovery.
منابع مشابه
Tissue- and Cell-Type Specific Transcriptome Profiling of Expanding Tomato Fruit Provides Insights into Metabolic and Regulatory Specialization and Cuticle Formation W OA
Antonio J. Matas,a Trevor H. Yeats,a Gregory J. Buda,a Yi Zheng,b Subhasish Chatterjee,c Takayuki Tohge,d Lalit Ponnala,e Avital Adato,f Asaph Aharoni,f Ruth Stark,c Alisdair R. Fernie,d Zhangjun Fei,b,g James J. Giovannoni,b,g and Jocelyn K.C. Rosea,1 a Department of Plant Biology, Cornell University, Ithaca, New York 14853 b Boyce Thompson Institute for Plant Research, Cornell University, Ith...
متن کاملComprehensive Tissue-Specific Transcriptome Analysis Reveals Distinct Regulatory Programs during Early Tomato Fruit Development.
Fruit formation and early development involve a range of physiological and morphological transformations of the various constituent tissues of the ovary. These developmental changes vary considerably according to tissue type, but molecular analyses at an organ-wide level inevitably obscure many tissue-specific phenomena. We used laser-capture microdissection coupled to high-throughput RNA seque...
متن کاملTissue-specific transcriptome profiling of the citrus fruit epidermis and subepidermis using laser capture microdissection
Most studies of the biochemical and regulatory pathways that are associated with, and control, fruit expansion and ripening are based on homogenized bulk tissues, and do not take into consideration the multiplicity of different cell types from which the analytes, be they transcripts, proteins or metabolites, are extracted. Consequently, potentially valuable spatial information is lost and the l...
متن کاملFine Mapping of a Gene (ER4.1) that Causes Epidermal Reticulation of Tomato Fruit and Characterization of the Associated Transcriptome
The hydrophobic cuticle that covers the surface of tomato (Solanum lycopersicum) fruit plays key roles in development and protection against biotic and abiotic stresses, including water loss, mechanical damage, UV radiation, pathogens, and pests. However, many details of the genes and regulatory mechanisms involved in cuticle biosynthesis in fleshy fruits are not well understood. In this study,...
متن کاملUnpuréeing the tomato: layers of information revealed by microdissection and high-throughput transcriptome sequencing.
Understanding the development of a complex structure such as a fruit provides both an interesting developmental model and an important task for agriculture, holding the potential of improving both product quality and human nutrition (Klee, 2010). For this task, the tomato (Solanum lycopersicum) fruit has proven a tractable model system, with complex metabolism and accessible genetics and genomi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Plant cell
دوره 23 11 شماره
صفحات -
تاریخ انتشار 2011